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KMID : 0363620070280020155
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Journal of Korean Oriental Medicine
2007 Volume.28 No. 2 p.155 ~ p.165
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Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells
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Jeong Mi-Young
Park Hi-Joon
Jeong Jee-Haeng
Kim Jin-Young
Kang Jun-Mo
Lee Na-Kyeong
Lim Sabina
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Abstract
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Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and NF-{kappa}B activity from RAW 264.7 cells, since NO and nuclear factor-{kappa}B (NF-{kappa}B) have been shown to be factors implicated in inflammatory disease.
Methods : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of NF-{kappa}B on western blot.
Results : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 {mu}g/ml. LPS-induced RAW 264.7 cells with AGE blocked inhibitory factor-{kappa}B{alpha} degradation.
Conclusion : This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of NF-{kappa}B activation in LPS-stimulated RAW 264.7 cells.
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KEYWORD
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Angelica gigas Nakai, RAW 264.7 cells, inflammation, nitric oxide, nuclear factor {kappa}B
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